Ultrasound-Guided Breath-Compensation in Single-Element Photoacoustic Imaging for Three-Dimensional Whole-Body Images of Mice

Photoacoustic imaging can be used to extract functional information at the molecular level for whole body of small animals in vivo . However, use a mechanical scanner image involves acquiring data period time which lead breathing-related artifacts. Thus, acquired three-dimensional cannot analyzed and visualized using two-dimensional projection rendering techniques unless respiratory distortions are minimized. In this study, an ultrasound-guided breath-compensation method photoacoustic macroscopy facilitate visualization analysis depth direction is presented. ultrasound mice were , then distorted skin layers compensated data. The extracted distortion parameters applied corresponding compensate motion. compensation was successfully visualize hemoglobin oxygen saturation by multispectral resulting images show that developed methodology various biomedical studies, including monitoring drug delivery, tumors, vasculature networks.